Solid State Fermentation of Citric Acid Production from Raja Banana Peels using Aspergillus niger (Effect of KH2PO4 as Phosphate Sources)

Authors

  • Nur Hidayat, MP. Central Laboratory of Life Science, University of Brawijaya
  • Anggraeni Dwi Khusumawati
  • Claudia Gadizza Perdani FTP UB

DOI:

https://doi.org/10.21776/ub.jfls.2019.003.02.03

Keywords:

Aspergillus niger, Citric acid, KH2PO4, Raja banana peels

Abstract

Citric acid is the organic acid widely used in food and pharmaceutical industries.The purpose of this study was to determine the effect of adding KH2PO4 as phosphate source of citric acid production from Raja banana peels using Aspergillus niger.The research is used Completely Randomized Design. Concentration of phosphate addition of  KH2PO4(0%; 0.5%; 1%; 1.5%; and 2%). The fermentation process is done for 7 days. Addition of concentration of KH2PO4 1,5% percentage highest citric acid content that is equal to 38,4% with pH 2,96 and total lowest reducing sugar 36,47 mg/g. This is reinforced by FTIR analysis indicating the presence of citric acid.

References

Abbas, N., Wardah, S., Sakhwat, A., Shahnaz, C., Sana, Ilahi. 2016. Citric Acid Production from Aspergillus niger using Banana Peel.International Journal of Scientific & Engineering Research. 7(1):1580-1583.

Ali, S., Ikram, H., Qadeer, M.,A., Javed, I. 2002. Production of Citric Acid byAspergillus Niger Using Cane Molasses in a Stirred Fermentor.Electronic Journal of Biotechnology. 5(3): 1717-3458.

Alsudani, Ali, A., Majid, K., A. 2015. Determine the Genotype of the Local Fungal Isolation Aspergillus niger 6 Producers of Citric Acid.International Journal of Advanced Research.3(7): 1058-1067.

Anastasisdis, S., Rehm, H.,J. 2005. Continous Production of Citric Acid Secretion by a High Specific pH Dependent Active Transport System in Yeast Candida oleophila, ATCC 20177.Electronic Journal of Biotechnology. 8(1): 26-42.

Angumeenal, A.,R., Venkappayya, D. 2005. Effect of Transition Metal Ions on The Metabolism of Aspergillus niger in The Production of Citric Acid With Molasses as Substrate.Journal of Scientific and Industrial Research. 64(1): 125-128.

Anhwange, B., A. 2008. Chemical Composition of Musa sapientum (Banana) Peels.Journal of Food Technology. 6(6): 263-266.

AOAC. 1995.Official Methods of Analysis 16th. Ed.The Association of Official Analytical Chemists. Washington DC.

Babayami, J., O., Dauda, K., T., Kayode, A., A., A., Nwude, D., O., Ajiboye, J., A., Essien, E., R., Abiona, O., O. 2010. Determination of Potash Alkali and Metal Content of Ashes Obtained from Peels of Some Varieties of Nigeria Grown Musa Species. Biotechnology Resources. 5(3): 1384-1392.

Badan Pusat Statistik. 2010. Kebutuhan Asam Sitrat di Indonesia 2010. Badan Pusat Statistik Indonesia. Jakarta.

Carlos, R., Vandenberghe, L.,P.,S., Rodrigues, C., Pandey, A. 2006. New Perspectives for Citric Acid Production and Application.Journal food Technology and Biotechnology. 44(2): 141-149.

Carolina, A., Abubakar, S., Iman, P., Maksum, Saadah, D., Rachman, Agus, S., Safri, I. 2015. Fermentasi Biak Rendam Molases dengan Apergillus niger untuk Produksi Asam Sitrat.Chimica et Natura Acta. 3(1): 25-29.

Cevrimli, B., S., Kariptas, E., Ciftci, H. 2009. Effect of Fermentation Conditionson Citric Acid Production from Beet Molasses by Aspergillus niger.Asian Journal of Chemistry. 21(1): 3211-3218.

Cevrimli, B., S., Yasar, A., Kariptas, E. 2010. Effect of O-Cresol, Resorcinol and Hydroquinone on Citric Acid Productivity by Aspergillus niger in Stirred Fermentor. Asian Journal of Chemistry. 22(1): 699-704.

Donald, L., Pavia, Gary, M.L., George, S.K., James, A.V. 2015. Introduction to Spectroscopy. Cengage Learning. Stamford.

Iqbal, Q. 2008. Quantification of Fungal Biomass Growth During Citric Acid Production by Aspergillus Niger on Expanded Clay Solid Substrate.Departement of Bioresource Engneering. McGill University. Québec.

Jinglan, W., Qijun, P., Wolfgang, A., and Mirjana, M. 2009. Model-Based Design of a Pilot-Scale Simulated Moving Bed for Purification of Citric Acid from Fermentation Broth.Journal of Chromatography. 1216(1): 8793-8805.

Kareem, S., O., Rahman, R., A. 2013. Utilization of Banana Peels for Citric Acid Production by Aspergillus niger.Agricultural and Biology Journal. 384(2): 2151-7517.

Kusumadewi, S. 2005. Pencarian Robot Atribut pada Multiple Attribute Decision Making (MADM) dengan Pendekatan Obyektif Menggunakan Algoritma Genetika. Jurnal Manajemen Informatika. 7(1): 1-9.

Liu, X., Xinfeng, W., Jiaxing, X., Jun, X., Jinshun, L., Tong, Z., Zhen, W., Yuanfang, D., Jianlong, H. 2015. Citric Acid Prouction by Yarrowia lipolytica SWJ-1b Using Corn Steep Liquor as a Source of Organic Nitrogen and Vitamins.Industrial Crops and Products. 78:154-160.

Manfaati, R. 2011. Pengaruh Komposisi Media Fermentasi terhadap Produksi Asam Sitrat oleh Aspergilus niger. Jurnal Fluida. 7(1): 23-27.

Max, B., Salgado, J.,M., Rodriguez, N., Cortes, S., Converti, A., Dominguez, J.,M. 2010. Biotechnological Production of Citric Acid.Brazilian Journal of Microbiology. 41(1): 862-875.

Mohapatra, D., Sabyasachi, M., Namrata, S. 2010. Banana and its by-Product Utilisation: an Overview.Journal of Scientific and Industrial Research. 69: 323-329.

Muredzi, P. 2015. Isolation of Citric Acid Producing Aspergillus niger Strain.Harare Institute Technology Publiser. Zimbawe.

Najafpour, G.,D. 2007. Biochemical Engineering and Biotechnology. Elesvier. United Kingdom.

Papagianni, Maria. 2007. Advances in Citric Acid Fermentation by Aspergillus niger: Biochemical Aspects, Membrane Transport and Modeling.Journal Biotechnology Advances. 25(1): 244-263.

Prado, F.,C., Vandenberghe, Woiciechowski, Leon, R., Soccol, C.,R. 2005. Citric Acid Production by Solid-State Fermentation on a Semi-Pilot Scale Using Different Percentages of Treated Cassava Bagasse. Journal of Chemical Engineering. 22(4): 547-555.

Sasmitaloka, K., S. 2017. Produksi Asam Sitrat oleh Aspergillus niger pada Kultivasi Media Cair. Jurnal Integrasi Proses. 6(3): 116-112.

Singh, Jyoti, B. S. Mehrotra. 2007. Mycological Production of Citric Acid in India IV. Role of sulphur and phosphorus compounds.Agricultural and Biology Journal. 75: 713-722.

Singh, Jyoti, B. S. Mehrotra. 2007. Mycological Production of Citric Acid in India IV. Role of sulphur and phosphorus compounds.Agricultural and Biology Journal. 75: 713-722.

Syamsuriputra, A., A., Tjandra, S., Ratih, K., Rita, F., Y. 2006. Kadar Air Substrat dan Konsentrasi Dedak Padi pada Produksi Asam Sitrat dari Ampas Tapioka Menggunakan Aspergillus niger ITBCCL74. Jurnal Tehnik Kimia. 32(1): 99–104.

Vandenberghe, L.P.S., Rodrigues, C., Medeiros, A.B.P., Soccol, C.R. 2017. Production and Application of Citric Acid • dalam Current Developments in Biotechnology and Bioengineering. Elsevier. Oxford.

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Published

2019-12-30

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