Identification and Antibacterial Activity Test of Lactic Acid Bacteria Isolated from Fermented Shrimp (Cincalok) Against Vibrio parahaemolyticus and Listeria monocytogenes
DOI:
https://doi.org/10.21776/ub.jfls.2019.003.01.02Keywords:
lactic acid bacteria, molecular identification, antibacterial activity, Vibrio parahaemolyticus, Listeria monocytogenesAbstract
Indonesia is a maritime country which has richness of sea products like fish, shrimp, squid, crab, and oyster. Nevertheless, all of those sea products can easily be contaminated by foodborne disease pathogenic bacterias such as Vibrio parahaemolyticus and Listeria monocytogenes. Those two pathogenic bacterias can cause serious illnesses in gastrointestinal tract such as diarhea, vomiting, and stomach cramp. The contamination of pathogenic bacteria can be prevented by lactic acid bacteria (LAB) metabolite compounds such as bacteriocin, organic acid, hydrogen peroxide, and diacetyl. Lactic acid bacteria was isolated from Indonesian traditional fermented shrimp called cincalok. The aim of this research is to do moleculary identify LAB isolates and antibacterial activity test in order to determine the species of LAB and to know the ability of LAB against pathogenic bacteria. Lactic acid bacteria from cincalok was identified by amplifying gen 16S rRNA through PCR colony method using primer LABFw and primer R16SRDNA-1492bac. Lactic acid bacteria from cincalok identified as Enterococcus sp. FTBUAJY01, Enterococcus sp. FTBUAJY02, Enterococcus sp. FTBUAJY03, Enterococcus durans strain FTBUAJY01, dan Enterococcus durans strain FTBUAJY02. Antibacterial activity test was conducted by agar well difusion method. It showed that the greatest inhibition ability of LAB from cincalok showed by Enterococcus sp. FTBUAJY02 against V. parahaemolyticus (0,529 ± 0,082 cm2) and Enterococcus durans strain FTBUAJY02 against L. monocytogenes (0,655 ± 0,090 cm2).
References
Dangkhaw, N., Maneerat, S. dan Sumpavapol, P. 2012. Characterization of lactic acid bacteria isolated from Kung-Som, a traditional fermented shrimp, in respect of their probiotic properties. International Conference on Nutrition and Food Sciences (39) : 121–125.
De Vuyst, L dan Vandamme, E. 1994. Antimicrobial Potential of Lactic Acid Bacteria. Bacteriocin of Lactic Acid Bacteria : Microbiology, Genetics, and Applications. Blackie Academic and Professional, London.
Gomes, B. C., Franco, B. D. dan Martinis, E. C. 2010. Dualistic Aspects of Enterococcus spp. in Foods. Formatex Research Center, Badajoz.
Hajep, P. dan Jinap, S. 2012. Fermented shrimp products as source of umami in Southeast Asia. Journal Nutrition Food Science 10 (6): 1–5.
Holt, J., Krieg, N., Sneath, P., Staley, J. dan Williams, S. 2000. Bergey's Manual of Determinative Bacteriology 9th edition. Lippincott Williams and Wilkins, Philadelphia.
Kanjanasopa, D., Pimpa, B. dan Chowpongpang, S. 2011. Occurrence of Vibrio parahaemolyticus in cockle (Anadara granosa) harvested from the south coast of Thailand. Songklanakarin Journal Science Technology 33 (3):295-300.
Keramas, G., Bang, D., Lund, M., Madsen, M., Bunkenborg, H., Telleman, P. dan Christensen, C. 2004. Use of culture, PCR analysis and DNA microarrays for detection of Campylobacter jejuni and Campylobacter coli from chicken faeces. Journal Clinical Microbiology (47) : 3985–3991.
Madigan, M. T. dan Martinko, J. M. 2006. Brock Biology of Microorganism 11th edition. Prentice Hall, New Jersey.
Magistrado, P., Carcia, M. dan Raymundo, A. 2001. Isolation and polymerase chain reaction-base detection of Campylobacter jejuni and Campylobacter coli from poultry in Philippines. International Journal Food Microbiology (70) : 194–206.
Nanasombat, S., Phunpruch, S. dan Jaichalad, T. 2012. Screening and identification of lactic acid bacteria from raw seafoods and Thai fermented seafood products for their potential use as starter cultures. Journal Science Technology 34 (3): 255–262.
Noordiana, N., Fatimah, A. dan Mun, A. 2013. Antibacterial agents produced by lactic acid bacteria isolated from Threadfin Salmon and Grass Shrimp. International Food Research Journal 20 (1): 117-124.
Peiris, W. 2005. Listeria monocytogenes, a food-borne pathogen. Thesis. Faculty of Veterinary Medicine and Animal Science, Swedish University of Agricultural Sciences, Swedia.
Sidharta, B. R. 2015. Budaya Bahari dari Nusantara Menuju Mataram Moderen. Gosyen Publishing, Yogyakarta.
Stevens, K. A., Sheldon, B. W., Klapes, N. A. dan Klaenhammer, T. R. 1991. Nisin treatment for inactivation of Salmonella species and other Gram negative bacteria. Applied and Environmental Microbiology (57) : 3613-3615.
